Thermodynamic Analysis of Weak Protein Interactions Using Sedimentation Equilibrium
نویسندگان
چکیده
منابع مشابه
Determination of membrane protein molecular weights and association equilibrium constants using sedimentation equilibrium and sedimentation velocity.
Regulated molecular interactions are essential for cellular function and viability, and both homo- and hetero-interactions between all types of biomolecules play important cellular roles. This chapter focuses on interactions between membrane proteins. Knowing both the stoichiometries and stabilities of these interactions in hydrophobic environments is a prerequisite for understanding how this c...
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Determination of the assembly state(s) of a membrane protein poses a difficult analytical problem. A powerful technique that is well adapted to meet this challenge is solution interaction analysis using analytical ultracentrifugation. Due to their hydrophobic nature, membrane proteins require detergent (or lipid) for solubilization in solution. Thus, a fundamental obstacle to be overcome in det...
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Analytical ultracentrifugation (AUC) is a versatile and powerful method for the quantitative analysis of macromolecules in solution. AUC has broad applications for the study of biomacromolecules in a wide range of solvents and over a wide range of solute concentrations. Three optical systems are available for the analytical ultracentrifuge (absorbance, interference, and fluorescence) that permi...
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ژورنال
عنوان ژورنال: Current Protocols in Protein Science
سال: 2014
ISSN: 1934-3655,1934-3663
DOI: 10.1002/0471140864.ps2013s77